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KMID : 0377519900150030265
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Chung-Ang Journal of Medicine
1990 Volume.15 No. 3 p.265 ~ p.278
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Roles of Superoxide Dismutase, Catalase and Superoxide Radical in Experimentally Induced Inflammation of Guinea Pig Skin
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Abstract
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In the experimentally induced inflammatory reaction by intradermal injection of´xanthme ox iciase (XOD) and hypoxanthine (HPX) into guinea pig skin, the author observed changes in ac-
} ytivities of superoxide dismutase- (SOD) and catalase, in the production of superoxide radical as well has in clinical and histopathological findings. The purpose was to investigate the roles of SOD, catalase and superoxide radical in the inflammatory reaction.
The results were as follows:
s 1. Clinically, the erythema reached a peak 3 hours after injection in both group A (intradermal-
´ injection of HPX and XOD) and group B (intradermal injection of mixture of´HPX + XOD and i
4 SOD + catalase) and disappeared slowly. The erythema was reduced by the combination of SOD ,
´mod catalase (group B).
r 2. Peaks of inflammatory reaction were histologically observed 3 hours after injection in both groups, being less marked in group B. These findings were consistent with clinical observation of
3. Activities of total SOD, Cu, Zn-SOD and Mn-SOD in group A were minimum 3 hours after .;),injection, and remained significantly reduced (p<0.01) up to 48 hours. In group B, SOD activities "´ere significantly higher (p<0.01) at the 3 hour point than group A. Actually, the reduction of SOD activity in group B was observed only at 24 hour point.
4. The time course of change in the catalase activity in group A was similar to that of SOD. In group B, the catalase activity was significantly higher than group A only at the 3 hour point after injection (p<0.05).
5. The time course of change in the superoxide radical production was opposite to those of SOD and catalase activities. In group A, the peak increase was observed 3 hours after injection, remain-?ng increased up to 24 hours (p<0.01). On the contrary in group B, the superoxide radical production was significantly reduced at 3 hour point (p<0.01).
These findings suggest that superoxide radicals play an important role in the initiation and pro
gression of inflmmma.tory reaction, and that SOD and catalase have protective roles in the inf]~-matory reaction.
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